Beilstein J. Nanotechnol.2021,12, 93–101, doi:10.3762/bjnano.12.8
electron irradiation-induced cross-linking of a self-assembled monolayer (SAM) of 4′-nitro-1,1′-biphenyl-4-thiol (NBPT) and second, purple membrane (PM) containing genetically modified bacteriorhodopsin (BR) carrying a C-terminal His-tag. The NBPT-CNM was further modified to carry nitrilotriaceticacid
(NTA) terminal groups for the interaction with the His-tagged PMs forming a quasi-monolayer of His-tagged PM on top of the CNM-NTA. The formation of the Ni-NTA/His-tag complex leads to the unidirectional orientation of PM on the CNM substrate. Electrophoretic sedimentation was employed to optimize the
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Figure 1:
WT PM deposited on NBPT CNM using drop-casting and electrophoretic sedimentation: (a, b) initial dr...
Beilstein J. Nanotechnol.2016,7, 544–553, doi:10.3762/bjnano.7.48
.7.48 Abstract Background: A versatile strategy for protein–surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriaceticacid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free
the adsorption of His6 on the Ni-free NTA layer, due to non specific interactions, from the formation of a neatly thicker His6 film induced by the Ni(II)-loading of the NTA SAM.
Keywords: His-tag; nitrilotriaceticacid (NTA); protein binding; self-assembled monolayers (SAMs); spectroscopic
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Figure 1:
(a) Chemical structure of the HS-C11-EG3-NTA (NTA1) molecule; (b) sketch of the steps of sample pre...